Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure.

Detaljer

Författare
  • Wael Awad
  • Gabriel Svensson Birkedal
  • Marjolein M G M Thunnissen
  • Katrin Mani
  • Derek T Logan
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Strukturbiologi
Originalspråkengelska
Sidor (från-till)2524-2533
TidskriftActa Crystallographica. Section D: Biological Crystallography
Volym69
UtgivningsnummerPt 12
StatusPublished - 2013
PublikationskategoriForskning
Peer review utfördJa