In vitro tumorigenic assay: colony forming assay for cancer stem cells

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKapitel samlingsverk

Abstract

Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.

Detaljer

Författare
  • Vijayalakshmi Rajendran
  • Mayur Vilas Jain
Enheter & grupper
Externa organisationer
  • University of Aberdeen
  • Linköping University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
  • Cancer och onkologi

Nyckelord

Originalspråkengelska
Titel på värdpublikationMethods in Molecular Biology
FörlagHumana Press
Sidor89-95
Antal sidor7
Volym1692
StatusPublished - 2018
PublikationskategoriForskning
Peer review utfördJa

Publikationsserier

NamnMethods in Molecular Biology
Volym1692
ISSN (tryckt)10643745