In vivo compartmentalization of functionally distinct, rapidly responsive antigen-specific T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The location and functional properties of antigen-specific memory T-cell populations in lymphoid and nonlymphoid compartments following DNA immunization or infection with Salmonella were investigated. Epitope-specific CD8+-T-cell expansion and retention during the memory phase were analyzed for DNA-immunized mice by use of a 5-h peptide restimulation assay. These data revealed that epitope-specific gamma interferon (IFN-{gamma})-positive CD8+ T cells occur at higher frequencies in the spleen, liver, and blood than in draining or peripheral lymph nodes during the expansion phase. Moreover, this distribution is maintained into long-term memory. The location and function of both CD4+ and CD8+ Salmonella-specific memory T cells in mice who were given a single dose of Salmonella enterica serovar Typhimurium was also quantitated by an ex vivo restimulation with bacterial lysate to detect the total Salmonella-specific memory pool. Mice immunized up to 6 months previously with S. enterica serovar Typhimurium had bacterium-specific CD4+ T cells that were capable of producing IFN-{gamma} or tumor necrosis factor alpha (TNF-{alpha}) at each site analyzed. Similar findings were observed for CD8+ T cells that were capable of producing IFN-{gamma}, while a much lower frequency and more restricted distribution were associated with TNF-{alpha}-producing CD8+ T cells. This study is the first to assess the frequencies, locations, and functions of both CD4+ and CD8+ memory T-cell populations in the same Salmonella-infected individuals and demonstrates the organ-specific functional compartmentalization of memory T cells after Salmonella infection.

Detaljer

Författare
  • Alun C. Kirby
  • Malin Sundquist
  • Mary Jo Wick
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Immunologi inom det medicinska området
Originalspråkengelska
Sidor (från-till)6390-6400
TidskriftInfection and Immunity
Volym72
Utgåva nummer11
StatusPublished - 2004
PublikationskategoriForskning
Peer review utfördJa

Nedladdningar

Ingen tillgänglig data