Increase in cardiac P2X1-and P2Y2-receptor mRNA levels in congestive heart failure

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Increase in cardiac P2X1-and P2Y2-receptor mRNA levels in congestive heart failure. / Hou, Mingyan; Malmsjö, Malin; Moller, Sebastian; Pantev, Emil; Bergdahl, Anders; Zhao, X H; Sun, X Y; Hedner, T; Edvinsson, Lars; Erlinge, David.

I: Life Sciences, Vol. 65, Nr. 11, 1999, s. 1195-1206.

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Hou, Mingyan ; Malmsjö, Malin ; Moller, Sebastian ; Pantev, Emil ; Bergdahl, Anders ; Zhao, X H ; Sun, X Y ; Hedner, T ; Edvinsson, Lars ; Erlinge, David. / Increase in cardiac P2X1-and P2Y2-receptor mRNA levels in congestive heart failure. I: Life Sciences. 1999 ; Vol. 65, Nr. 11. s. 1195-1206.

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TY - JOUR

T1 - Increase in cardiac P2X1-and P2Y2-receptor mRNA levels in congestive heart failure

AU - Hou, Mingyan

AU - Malmsjö, Malin

AU - Moller, Sebastian

AU - Pantev, Emil

AU - Bergdahl, Anders

AU - Zhao, X H

AU - Sun, X Y

AU - Hedner, T

AU - Edvinsson, Lars

AU - Erlinge, David

PY - 1999

Y1 - 1999

N2 - We wanted to study the expression of P2-receptors at the mRNA-level in the heart and if it is affected by congestive heart failure (CHF). To quantify the P2 receptor mRNA-expression we used a competitive RT-PCR protocol which is based on an internal RNA standard. The P2 receptor mRNA-expression was quantified in hearts from CHF rats and compared to sham-operated rats. Furthermore, the presence of receptor mRNA was studied in the myocardium from patients with heart failure. In the sham operated rats the G-protein coupled P2Y-receptors were expressed at a higher level than the ligand gated ion-channel receptor (P2X1). Among the P2Y-receptors the P2Y6-receptor was most abundantly expressed (P2Y6 > P2Y1 > P2Y2 = P2Y4 > P2X1). A prominent change was seen for the P2X1- and P2Y2-receptor mRNA levels which were increased 2.7-fold and 4.7-fold respectively in the myocardium from the left ventricle of CHF-rats. In contrast, the P2Y1-, P2Y4- and P2Y6-receptor mRNA levels were not significantly altered in CHF rats. In human myocard the P2X1-, P2Y1-, P2Y2-, P2Y6- and P2Y11-receptors were detected by RT-PCR in both right and left atria and ventricles, while the P2Y4-receptor band was weak or absent. In conclusion, most of the studied P2-receptors were expressed in both rat and human hearts. Furthermore, the P2X1- and P2Y2-receptor mRNA were upregulated in CHF, suggesting a pathophysiological role for these receptors in the development of heart failure.

AB - We wanted to study the expression of P2-receptors at the mRNA-level in the heart and if it is affected by congestive heart failure (CHF). To quantify the P2 receptor mRNA-expression we used a competitive RT-PCR protocol which is based on an internal RNA standard. The P2 receptor mRNA-expression was quantified in hearts from CHF rats and compared to sham-operated rats. Furthermore, the presence of receptor mRNA was studied in the myocardium from patients with heart failure. In the sham operated rats the G-protein coupled P2Y-receptors were expressed at a higher level than the ligand gated ion-channel receptor (P2X1). Among the P2Y-receptors the P2Y6-receptor was most abundantly expressed (P2Y6 > P2Y1 > P2Y2 = P2Y4 > P2X1). A prominent change was seen for the P2X1- and P2Y2-receptor mRNA levels which were increased 2.7-fold and 4.7-fold respectively in the myocardium from the left ventricle of CHF-rats. In contrast, the P2Y1-, P2Y4- and P2Y6-receptor mRNA levels were not significantly altered in CHF rats. In human myocard the P2X1-, P2Y1-, P2Y2-, P2Y6- and P2Y11-receptors were detected by RT-PCR in both right and left atria and ventricles, while the P2Y4-receptor band was weak or absent. In conclusion, most of the studied P2-receptors were expressed in both rat and human hearts. Furthermore, the P2X1- and P2Y2-receptor mRNA were upregulated in CHF, suggesting a pathophysiological role for these receptors in the development of heart failure.

KW - P2Y

KW - P2X

KW - receptor

KW - quantitative RT-PCR

KW - congestive heart failure

U2 - 10.1016/S0024-3205(99)00353-7

DO - 10.1016/S0024-3205(99)00353-7

M3 - Article

VL - 65

SP - 1195

EP - 1206

JO - Life Sciences

JF - Life Sciences

SN - 1879-0631

IS - 11

ER -