Increased expression of nestin in the major pelvic ganglion following cavernous nerve injury

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In an effort to identify neuronal repair mechanisms of the major pelvic ganglion (MPG), we evaluated changes in the expression of nestin, an intermediate filament protein and neural stem cell marker following cavernous nerve crush injury (CNI). We utilized two groups of Sprague Dawley rats: (i) sham and (ii) bilateral CNI. Erectile responses to cavernous nerve stimulation (CNS) were determined at 48h in a subset of rats. The MPG was isolated and removed at 48h after CNI, and nestin immunolocalization, protein levels and RNA expression were evaluated. At 48 h, erectile responses to CNS in CNI rats were substantially reduced (P<0.05; similar to 70% decrease in intra-cavernous pressure/mean arterial pressure) compared with sham surgery controls. This coincided with a dramatic 10-fold increase (P<0.05) in nestin messenger RNA expression and protein levels in the MPG of rats with CNI. Immunoflourescence microscopy demonstrated that nestin upregulation after CNI occurred within the ganglion cell bodies and nerve fibers of the MPG. In conclusion, CNI induces nestin in the MPG. These data suggest that nestin may be involved in the regenerative process of the cavernous nerve following crush injury. International Journal of Impotence Research (2012) 24, 84-90; doi:10.1038/ijir.2011.50; published online 13 October 2011

Detaljer

Författare
  • O. Kutlu
  • A. E. Ross
  • E. M. Schaeffer
  • C. Gratzke
  • C. G. Stief
  • T. D. Strong
  • A. L. Burnett
  • Petter Hedlund
  • T. J. Bivalacqua
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Reproduktionsmedicin och gynekologi

Nyckelord

Originalspråkengelska
Sidor (från-till)84-90
TidskriftInternational Journal of Impotence Research
Volym24
Utgivningsnummer2
StatusPublished - 2012
PublikationskategoriForskning
Peer review utfördJa