Increasing amperometric biosensor sensitivity by length fractionated single-walled carbon nanotubes.

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In this work the sensitivity-increasing effect of single-walled carbon nanotubes (SWCNTs) in amperometric biosensors, depending on their average length distribution, was studied. For this purpose the SWCNTs were oxidatively shortened and subsequently length separated by size exclusion chromatography. Transmission electron micrographs of different fractions of SWCNTs were collected. Diaphorase "wired" to an osmium redox polymer was blended with the shortened SWCNTs of different lengths. Depending on the average length of the SWCNTs the sensitivity of the amperometric biosensor model system towards oxidation of 1,4-dihydronicotinamide adenine dinucleotide (NADH) was increased by a factor of five. The best performance was achieved with SWCNTs of medium length. The linear range for NADH detection was between 5muM and 7mM, the maximum sensitivity was 47nAmuM(-1)cm(-2), and the detection limit was 1muM. The biosensor exhibited excellent electrocatalytic properties. Even at relatively high NADH concentrations the oxidative current was limited by the diffusion rate of NADH.


  • Federico Tasca
  • Lo Gorton
  • Jakob Wagner
  • Gilbert Nöll
Enheter & grupper

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Analytisk kemi
  • Kemi


Sidor (från-till)272-278
TidskriftBiosensors & Bioelectronics
StatusPublished - 2008
Peer review utfördJa