Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A method is described for quantification of the beef tenderness marker, calpastatin, in meat samples by amperometric detection. Using a novel bovine recombinant partial calpastatin protein as standard antigen a low detection limit of 0.2 ng/mL was achieved. The influence of the complex matrix was minimised by heat pretreatment and dilution of the samples prior to detection of calpastatin. The relative error between the direct sample measurement and standard addition methods was 5.89%, confirming the accuracy of the developed amperometric immunoassay. (C) 2012 Elsevier Ltd. All rights reserved.

Detaljer

Författare
  • Kinga Zor
  • Katarzyna Dymek
  • Roberto Ortiz
  • Audrey Faure
  • Ebru Saatci
  • Lo Gorton
  • Ronald Bardsley
  • Mihaela Nistor
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Livsmedelsvetenskap

Nyckelord

Originalspråkengelska
Sidor (från-till)598-603
TidskriftFood Chemistry
Volym133
Utgivningsnummer2
StatusPublished - 2012
PublikationskategoriForskning
Peer review utfördJa