Induction of S100A9 homodimer formation in vivo

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

We show here that increased S100A8 and S100A9 protein expression is induced in spleen of animals with active inflammation or with inoculated tumors. In tumor bearing animals an increased expression was also detected in the lung. To further analyze the induced proteins, we performed chemical cross-linking followed by Western blotting. We observed in protein extracts from spleen that both S100A8/S100A9 heterodimers as well as S100A9 homodimers were formed, both after tumor and inflammatory challenge. The cellular source for S100A9 homodimers were CD11b+GR1+ cells. S100A9 homodimers were also secreted into the extracellular space. Lastly, in the spleen from normal and tumor bearing animals cells expressing relatively higher levels of S100A9 compared to S100A8 could be observed by immunohistochemistry. Taken together, these data show that the biologically potent dimeric form of S100A9 is induced in vivo in situations of tumor burden or inflammatory challenge.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
  • Immunologi

Nyckelord

Originalspråkengelska
Sidor (från-till)564-568
Antal sidor5
TidskriftBiochemical and Biophysical Research Communications
StatusPublished - 2018 jun
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Tahvili, S., 2018, Lund: Lund University, Faculty of Medicine. 72 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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