Influence of the reaction medium on the product distribution of peroxidase-catalysed oxidation of p-cresol

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Abstract

p-Cresol was oxidized by hydrogen peroxide in a reaction catalysed by horseradish peroxidase and the low molecular weight products were investigated. In aqueous media Pummerer's ketone (I) was the dominating product but in organic media the product distribution was quite different; 2,2'-dihydroxy-5,5'-dimethyldiphenyl (II) was the main low molecular weight product. Similar product distributions were obtained with peroxidase adsorbed on a solid support and suspended in toluene and with peroxidase solubilized in a microemulsion containing the same solvent. The best selectivity for the formation of (II) was obtained when the enzyme was adsorbed on Celite and suspended in water-saturated chloroform with 0.5% (v/v) extra water added. The yield of low molecular weight products in this case was 28%; of this fraction, 95% was (II).

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Lund University
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokatalys och enzymteknik
Originalspråkengelska
Sidor (från-till)455-458
Antal sidor4
TidskriftApplied Microbiology and Biotechnology
Volym33
Utgåva nummer4
StatusPublished - 1990 jul 1
PublikationskategoriForskning
Peer review utfördJa