Instantaneous isotropic volumetric imaging of fast biological processes

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.

Detaljer

Författare
  • Nils Wagner
  • Nils Norlin
  • Jakob Gierten
  • Gustavo de Medeiros
  • Bálint Balázs
  • Joachim Wittbrodt
  • Lars Hufnagel
  • Robert Prevedel
Externa organisationer
  • Karolinska Institute
  • Friedrich Miescher Institute for Biomedical Research
  • University Hospital Heidelberg
  • Heidelberg University
  • European Molecular Biology Laboratory Heidelberg
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)497-500
Antal sidor4
TidskriftNature Methods
Volym16
Utgåva nummer6
StatusPublished - 2019 jun
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa