Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Klinisk medicin
  • Läkemedelskemi
Originalspråkengelska
Sidor (från-till)1790-1796
TidskriftLab on a Chip
Volym13
Utgivningsnummer9
StatusPublished - 2013
PublikationskategoriForskning
Peer review utfördJa

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Relaterad forskningsoutput

Klara Petersson, 2018 feb 20, 1 uppl. Lund: Department of Biomedical Engineering, Lund university. 159 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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