Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.

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Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. / Ahmad Tajudin, Asilah; Petersson, Klara; Lenshof, A; Swärd-Nilsson, A-M; Åberg, Lena; Marko-Varga, György; Malm, Johan; Lilja, Hans; Laurell, Thomas.

I: Lab on a Chip, Vol. 13, Nr. 9, 2013, s. 1790-1796.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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TY - JOUR

T1 - Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.

AU - Ahmad Tajudin, Asilah

AU - Petersson, Klara

AU - Lenshof, A

AU - Swärd-Nilsson, A-M

AU - Åberg, Lena

AU - Marko-Varga, György

AU - Malm, Johan

AU - Lilja, Hans

AU - Laurell, Thomas

PY - 2013

Y1 - 2013

N2 - On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.

AB - On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%.

U2 - 10.1039/c3lc41269e

DO - 10.1039/c3lc41269e

M3 - Article

VL - 13

SP - 1790

EP - 1796

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

T2 - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0189

IS - 9

ER -