Integrated Brain Atlas for Unbiased Mapping of Nervous System Effects Following Liraglutide Treatment

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Light Sheet Fluorescence Microscopy (LSFM) of whole organs, in particular the brain, offers a plethora of biological data imaged in 3D. This technique is however often hindered by cumbersome non-automated analysis methods. Here we describe an approach to fully automate the analysis by integrating with data from the Allen Institute of Brain Science (AIBS), to provide precise assessment of the distribution and action of peptide-based pharmaceuticals in the brain. To illustrate this approach, we examined the acute central nervous system effects of the glucagon-like peptide-1 (GLP-1) receptor agonist liraglutide. Peripherally administered liraglutide accessed the hypothalamus and brainstem, and led to activation in several brain regions of which most were intersected by projections from neurons in the lateral parabrachial nucleus. Collectively, we provide a rapid and unbiased analytical framework for LSFM data which enables quantification and exploration based on data from AIBS to support basic and translational discovery.

Detaljer

Författare
  • Casper Bo Gravesen Salinas
  • Tess Tsai-Hsiu Lu
  • Sanaz Gabery
  • Kasper Marstal
  • Tomas Alanentalo
  • Aaron Jeffrey Mercer
  • Anda Cornea
  • Knut Conradsen
  • Jacob Hecksher-Sørensen
  • Anders Bjorholm Dahl
  • Lotte Bjerre Knudsen
  • Anna Secher
Enheter & grupper
Externa organisationer
  • Technical University of Denmark
  • Novo Nordisk A/S
  • Erasmus University Medical Center
  • Novo Nordisk, Inc., US
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Bioinformatik (beräkningsbiologi)
  • Neurovetenskaper
Originalspråkengelska
Artikelnummer10310
Antal sidor12
TidskriftScientific Reports
Volym8
Utgivningsnummer1
StatusPublished - 2018 jul 9
PublikationskategoriForskning
Peer review utfördJa