Interaction of Ferrocene Mediators with Gluconobacter oxydans Immobilized Whole Cells and Membrane Fractions in Oxidation of Ethanol

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Gluconobacter oxydans whole cells and membrane fractions in combination with ferrocene mediators were used to study oxidation of ethanol. The efficiency of mediator?enzyme interaction was assessed by the ratio of maximum current to the apparent Michaelis constant (Imax/KM) for saturating mediator concentrations. The bioelectrocatalytic processes were found to be more efficient with membrane fractions. The highest Imax/KM value of 120 and 3200 mu A g/mol for, respectively, cells and fractions was obtained for ferrocene carboxylic acid. In test measurements of biological oxygen demand for rye distillers grains, the values obtained by a biosensor based on Gluconobacter membrane fractions and ferrocene were found to correlate with the reference data.

Detaljer

Författare
  • E. Yu Indzhgiya
  • O. N. Ponamoreva
  • V. A. Alferov
  • A. N. Reshetilov
  • Lo Gorton
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biologiska vetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)924-930
TidskriftElectroanalysis
Volym24
Utgåva nummer4
StatusPublished - 2012
PublikationskategoriForskning
Peer review utfördJa