Interactive model building in neutron macromolecular crystallography

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKapitel samlingsverk

Abstract

This chapter aims to give an overview of the process of interactive model building in macromolecular neutron crystallography for the researcher transitioning from X-ray crystallography alone. The two most popular programs for refinement and model building, phenix.refine and Coot, respectively, are used as examples, and familiarity with the programs is assumed. Some work-arounds currently required for proper communication between the programs are described. We also discuss the appearance of nuclear density maps and how this differs from that of electron density maps. Advice is given to facilitate deposition of jointly refined neutron/X-ray structures in the Protein Data Bank.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokemi och molekylärbiologi
  • Strukturbiologi

Nyckelord

Originalspråkengelska
Titel på värdpublikationMethods in Enzymology
FörlagAcademic Press
StatusE-pub ahead of print - 2019 dec 20
PublikationskategoriForskning
Peer review utfördJa

Publikationsserier

NamnMethods in Enzymology
ISSN (tryckt)0076-6879
ISSN (elektroniskt)1557-7988