Ion exchange chromatography of glucosamine and galactosamine in microgram amounts with quantitative determination and specific radioactivity assay

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A rapid method for the separation and quantitative determination, including radioassay, of glucosamine and galactosamine in microgram amounts is described. It is based upon the use of a column of Aminex A-5 ion exchange resin eluted with a phosphate buffer at pH 7.00 and 60 °C. From each fraction half the volume is used for a scaled down Elson-Morgan procedure and other half for liquid scintillation counting. Amounts of about 0.01-1 μmole of hexosamine may be separated and determined.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Karolinska Institute
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Ortopedi
Originalspråkengelska
Sidor (från-till)411-417
Antal sidor7
TidskriftBiochimica et Biophysica Acta. General Subjects
Volym264
Utgåva nummer3
StatusPublished - 1972 maj 16
PublikationskategoriForskning
Peer review utfördJa