IRF8 Transcription Factor Controls Survival and Function of Terminally Differentiated Conventional and Plasmacytoid Dendritic Cells, Respectively

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Interferon regulatory factor-8 (IRF8) has been proposed to be essential for development of monocytes, plasmacytoid dendritic cells (pDCs) and type 1 conventional dendritic cells (cDC1s) and remains highly expressed in differentiated DCs. Transcription factors that are required to maintain the identity of terminally differentiated cells are designated “terminal selectors.” Using BM chimeras, conditional Irf8fl/fl mice and various promotors to target Cre recombinase to different stages of monocyte and DC development, we have identified IRF8 as a terminal selector of the cDC1 lineage controlling survival. In monocytes, IRF8 was necessary during early but not late development. Complete or late deletion of IRF8 had no effect on pDC development or survival but altered their phenotype and gene-expression profile leading to increased T cell stimulatory function but decreased type 1 interferon production. Thus, IRF8 differentially controls the survival and function of terminally differentiated monocytes, cDC1s, and pDCs.

Detaljer

Författare
  • Dorine Sichien
  • Charlotte L. Scott
  • Liesbet Martens
  • Matthias Vanderkerken
  • Sofie Van Gassen
  • Maud Plantinga
  • Thorsten Joeris
  • Sofie De Prijck
  • Leen Vanhoutte
  • Manon Vanheerswynghels
  • Gert Van Isterdael
  • Wendy Toussaint
  • Filipe Branco Madeira
  • Karl Vergote
  • Björn E. Clausen
  • Hamida Hammad
  • Marc Dalod
  • Yvan Saeys
  • Bart N. Lambrecht
  • Martin Guilliams
Enheter & grupper
Externa organisationer
  • Ghent University
  • Ghent University Hospital
  • Technical University of Denmark
  • University of Mainz
  • Erasmus University Medical Center
  • Aix-Marseille University
  • Centre d'Immunologie Marseille Luminy
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Immunologi inom det medicinska området

Nyckelord

Originalspråkengelska
Sidor (från-till)626-640
Antal sidor15
TidskriftImmunity
Volym45
Utgivningsnummer3
StatusPublished - 2016 sep 20
PublikationskategoriForskning
Peer review utfördJa