Isolation and Characterization of Shiga Toxin-Associated Microvesicles

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKapitel samlingsverk

Abstract

Microvesicles are shed from cell surfaces during infectious or inflammatory conditions and may contribute to the pathogenesis of disease. During Shiga toxin-producing Escherichia coli (STEC) infection, microvesicles are released from blood cells. These microvesicles play a part in inflammation, thrombosis, hemolysis, and the transfer of the main virulence factor of STEC strains, Shiga toxin, to target organ cells. This chapter describes how to isolate blood cell- and cell culture-derived microvesicles from plasma or cell culture medium, respectively, and how to characterize these microvesicles by various methods, with special focus on Shiga toxin-associated microvesicles.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Pediatrik
  • Cell- och molekylärbiologi

Nyckelord

Originalspråkengelska
Titel på värdpublikationShiga Toxin-Producing E. coli
FörlagHumana Press
Sidor207-228
Antal sidor22
ISBN (tryckt)978-1-0716-1339-9
StatusPublished - 2021
PublikationskategoriForskning
Peer review utfördJa

Publikationsserier

NamnMethods in Molecular Biology
Volym2291
ISSN (tryckt)1064-3745
ISSN (elektroniskt)1940-6029