Isolation and characterization of water-soluble hemicelluloses from flax shive,

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Isolation and characterization of water-soluble hemicelluloses from flax shive, / Jacobs, Anna; Palm, Magnus; Zacchi, Guido; Dahlman, Olof.

I: Carbohydrate Research, Vol. 338, Nr. 18, 2003, s. 1869-1876.

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Jacobs, Anna ; Palm, Magnus ; Zacchi, Guido ; Dahlman, Olof. / Isolation and characterization of water-soluble hemicelluloses from flax shive,. I: Carbohydrate Research. 2003 ; Vol. 338, Nr. 18. s. 1869-1876.

RIS

TY - JOUR

T1 - Isolation and characterization of water-soluble hemicelluloses from flax shive,

AU - Jacobs, Anna

AU - Palm, Magnus

AU - Zacchi, Guido

AU - Dahlman, Olof

PY - 2003

Y1 - 2003

N2 - Partially depolymerized, water-soluble hemicelluloses were solubilized from flax shive employing hydrothermal microwave treatment and thereafter subjected to ion-exchange chromatography, enzymatic purification and/or size-exclusion chromatography (SEC). The oligo- and polysaccharide fractions thus obtained were characterized with respect to molar mass, molar mass distribution, degree of polymerization (DP) and degree of substitution with acetyl moieties (DSAc) by employing SEC in combination with MALDI-TOF mass spectrometry. The major portion of the water-soluble flax hemicellulose consisted of an O-acetyl-4-O-methylglucuronoxylan exhibiting a DPp value (i.e., peak-average DP) of 28. When the DSAc for this O-acetyl-4-O-methylglucuronoxylan was calculated on the basis of the MALDI-MS spectra obtained without and following deacetylation, a value of 0.7 was obtained. In addition, an O-acetyl-glucomannan (DPp=9, DS=0.4) and minor quantities of small neutral O-acetyl-xylooligosaccharides were also isolated from the mixture of water-soluble hemicelluloses released from the flax shive by microwave treatment.

AB - Partially depolymerized, water-soluble hemicelluloses were solubilized from flax shive employing hydrothermal microwave treatment and thereafter subjected to ion-exchange chromatography, enzymatic purification and/or size-exclusion chromatography (SEC). The oligo- and polysaccharide fractions thus obtained were characterized with respect to molar mass, molar mass distribution, degree of polymerization (DP) and degree of substitution with acetyl moieties (DSAc) by employing SEC in combination with MALDI-TOF mass spectrometry. The major portion of the water-soluble flax hemicellulose consisted of an O-acetyl-4-O-methylglucuronoxylan exhibiting a DPp value (i.e., peak-average DP) of 28. When the DSAc for this O-acetyl-4-O-methylglucuronoxylan was calculated on the basis of the MALDI-MS spectra obtained without and following deacetylation, a value of 0.7 was obtained. In addition, an O-acetyl-glucomannan (DPp=9, DS=0.4) and minor quantities of small neutral O-acetyl-xylooligosaccharides were also isolated from the mixture of water-soluble hemicelluloses released from the flax shive by microwave treatment.

U2 - 10.1016/S0008-6215(03)00308-2

DO - 10.1016/S0008-6215(03)00308-2

M3 - Article

VL - 338

SP - 1869

EP - 1876

JO - Carbohydrate Research

JF - Carbohydrate Research

SN - 1873-426X

IS - 18

ER -