Label-free enrichment of cancer cells using negative selection (depletion) of wbcs with elastomeric negative acoustic contrast particles and acoustophoresis

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKonferenspaper i proceeding

Abstract

In an effort to bioengineer elastomeric particles (EPs), with negative acoustic contrast factor, to capture cells, we have successfully functionalized EPs with anti-human CD45 antibodies that enable capturing of WBCs from human RBC lysed blood samples. We demonstrate that the negative selection of WBCs with EPs and acoustophoresis can be employed to reduce WBC contamination while enriching label-free prostate cancer cells (DU145) within the central fraction of our acoustophoresis chip.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cancer och onkologi

Nyckelord

Originalspråkengelska
Titel på värdpublikation18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
FörlagChemical and Biological Microsystems Society
Sidor2540-2541
Antal sidor2
ISBN (elektroniskt)9780979806476
ISBN (tryckt)9781634396974
StatusPublished - 2014
PublikationskategoriForskning
Peer review utfördJa
Evenemang18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, USA
Varaktighet: 2014 okt 262014 okt 30
Konferensnummer: 18

Konferens

Konferens18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
LandUSA
OrtSan Antonio
Period2014/10/262014/10/30