Label-free separation of leukocyte subpopulations using high throughput multiplex acoustophoresis

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Bibtex

@article{4ee3cab5113849fbbe40488cf1b2db37,
title = "Label-free separation of leukocyte subpopulations using high throughput multiplex acoustophoresis",
abstract = "Multiplex separation of mixed cell samples is required in a variety of clinical and research applications. Herein, we present an acoustic microchip with multiple outlets and integrated pre-alignment channel to enable high performance and label-free separation of three different cell or particle fractions simultaneously at high sample throughput. By implementing a new cooling system for rigorous temperature control and minimal acoustic energy losses, we were able to operate the system isothermally and sort suspensions of 3, 5 and 7 μm beads with high efficiencies (>95.4{\%}) and purities (>96.3{\%}) at flow rates up to 500 μL min -1 corresponding to a throughput of ∼2.5 × 10 6 beads per min. Also, human viable white blood cells were successfully fractionated into lymphocytes, monocytes and granulocytes with high purities of 96.5 ± 1.6{\%}, 71.8 ± 10.1{\%} and 98.8 ± 0.5{\%}, respectively, as well as high efficiencies (96.8 ± 3.3{\%}, 66.7 ± 3.2{\%} and 99.0 ± 0.7{\%}) at flow rates up to 100 μL min -1 (∼100000 cells per min). By increasing the flow rate up to 300 μL min -1 (∼300000 cells per min) both lymphocytes and granulocytes were still recovered with high purities (92.8 ± 1.9{\%}, 98.2 ± 1.0{\%}), whereas the monocyte purity decreased to 20.9 ± 10.3{\%}. The proposed isothermal multiplex acoustophoresis platform offers efficient fractionation of complex samples in a label-free and continuous manner at thus far unreached high sample throughput rates.",
author = "Anke Urbansky and Franziska Olm and Stefan Scheding and Thomas Laurell and Andreas Lenshof",
year = "2019",
month = "1",
day = "1",
doi = "10.1039/c9lc00181f",
language = "English",
volume = "19",
pages = "1406--1416",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0189",
publisher = "Royal Society of Chemistry",
number = "8",

}