Light-induced absorption changes in etiolated Coleoptiles
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Blue (or green) light induced reversible and irreversible absorption changes in etiolated wheat (Triticum aestivum) coleoptiles. Immediately after a 10 s pulse of blue light, there was an absorbance increase at 440 nm and a decrease at 420 nm. The absorbance at 440 nm returned to the original level in a biphasic way with 1st-order rate constants of 0.05 s-1 and 0.006 s-1 at 25.degree. C. The change at 440 nm was partly, but not completely, inhibited by iodide. In the 500-600 nm region, complex changes occurred, indicating the participation of at least 2 different cytochromes, one of which was oxidized during a 10 s light pulse and the other oxidized more slowly during the following dark period.