Linoleic acid esters of hydroxy linoleic acids are anti-inflammatory lipids found in plants and mammals

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biologically active lipids. Here we identify the linoleic acid ester of 13-hydroxy linoleic acid (13-LAHLA) as an anti-inflammatory lipid. An oat oil fraction and FAHFA-enriched extract from this fraction showed anti-inflammatory activity in a lipopolysaccharide-induced cytokine secretion assay. Structural studies identified three LAHLA isomers (15-, 13-, and 9-LAHLA) as being the most abundant FAHFAs in the oat oil fraction. Of these LAHLAs, 13-LAHLA is the most abundant LAHLA isomer in human serum after ingestion of liposomes made of fractionated oat oil, and it is also the most abundant endogenous LAHLA in mouse and human adipose tissue. As a result, we chemically synthesized 13-LAHLA for biological assays. 13-LAHLA suppresses lipopolysaccharide-stimulated secretion of cytokines and expression of pro-inflammatory genes. These studies identify LAHLAs as an evolutionarily conserved lipid with anti-inflammatory activity in mammalian cells.

Detaljer

Författare
  • Matthew J. Kolar
  • Srihari Konduri
  • Tina Chang
  • Huijing Wang
  • Clare McNerlin
  • Lena Ohlsson
  • Magnus Härröd
  • Dionicio Siegel
  • Alan Saghatelian
Enheter & grupper
Externa organisationer
  • University of California, San Diego
  • Salk Institute for Biological Studies
  • Härröd Research AB
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Läkemedelskemi
Originalspråkengelska
Sidor (från-till)10698-10707
Antal sidor10
TidskriftJournal of Biological Chemistry
Volym294
Utgåva nummer27
StatusPublished - 2019 jul 5
PublikationskategoriForskning
Peer review utfördJa