Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Medicin och hälsovetenskap
Originalspråkengelska
Sidor (från-till)1399-1403
TidskriftThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Volym34
Utgåva nummer11
StatusPublished - 1986
PublikationskategoriForskning
Peer review utfördJa