Long-Term persistence of human donor alveolar macrophages in lung transplant recipients

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Background Alveolar macrophages (AMFs) are critical regulators of lung function, and may participate in graft rejection following lung transplantation. Recent studies in experimental animals suggest that most AMFs are self-maintaining cells of embryonic origin, but knowledge about the ontogeny and life span of human AMFs is scarce. Methods To follow the origin and longevity of AMFs in patients with lung transplantation for more than 100â €..weeks, we obtained transbronchial biopsies from 10 gender-mismatched patients with lung transplantation. These were subjected to combined in situ hybridisation for X/Y chromosomes and immunofluorescence staining for macrophage markers. Moreover, development of AMFs in humanised mice reconstituted with CD34+ umbilical cord-derived cells was assessed. Results The number of donor-derived AMFs was unchanged during the 2â €..year post-Transplantation period. A fraction of the AMFs proliferated locally, demonstrating that at least a subset of human AMFs have the capacity to self-renew. Lungs of humanised mice were found to abundantly contain populations of human AMFs expressing markers compatible with a monocyte origin. Moreover, in patients with lung transplantation we found that recipient monocytes seeded the alveoli early after transplantation, and showed subsequent phenotypical changes consistent with differentiation into proliferating mature AMFs. This resulted in a stable mixed chimerism between donor and recipient AMFs throughout the 2-year period. Conclusions The finding that human AMFs are maintained in the lung parenchyma for several years indicates that pulmonary macrophage transplantation can be a feasible therapeutic option for patients with diseases caused by dysfunctional AMFs. Moreover, in a lung transplantation setting, long-Term persistence of donor AMFs may be important for the development of chronic graft rejection.

Detaljer

Författare
  • Ibon Eguíluz-Gracia
  • Hans Henrik Lawaetz Schultz
  • Liv I B Sikkeland
  • Elena Danilova
  • Are M. Holm
  • Cornelis J H Pronk
  • William W. Agace
  • Martin Iversen
  • Claus Andersen
  • Frode L. Jahnsen
  • Espen S. Baekkevold
Enheter & grupper
Externa organisationer
  • Copenhagen University Hospital
  • University of Oslo
  • Oslo university hospital
  • Lund University
  • University of Copenhagen
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Lungmedicin och allergi

Nyckelord

Originalspråkengelska
Sidor (från-till)1006-1011
Antal sidor6
TidskriftThorax
Volym71
Utgivningsnummer11
StatusPublished - 2016 nov 1
PublikationskategoriForskning
Peer review utfördJa