Loss of Cdk2 and cyclin A2 impairs cell proliferation and tumorigenesis

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Cell-cycle inhibition has yet to offer a generally effective approach to cancer treatment, but a full evaluation of different combinations of cell-cycle inhibitors has not been evaluated. Cyclin A2, a core component of the cell cycle, is often aberrantly expressed in cancer where it may impact cell proliferation. In this study, we investigated the role of cyclin A2 in tumorigenesis using a conditional genetic knockout mouse model. Cyclin A2 deletion in oncogene-transformed mouse embryonic fibroblasts (MEF) suppressed tumor formation in immunocompromised mice. These findings were confirmed in mice with cyclin A2-deficient hepatocytes, where a delay in liver tumor formation was observed. Because cyclin A2 acts in complex with Cdk2 in the cell cycle, we explored a hypothesized role for Cdk2 dysregulation in this effect through conditional deletions of both genes. In oncogene-transformed MEFs lacking both genes, tumor formation was strongly suppressed in a manner associated with decreased proliferation, premature senescence, and error-prone recovery from serum deprivation after immortalization. Whereas loss of cyclin A2 led to a compensatory increase in Cdk1 activity, this did not occur with loss of both Cdk2 and cyclin A2. Our work offers a rationale to explore combinations of Cdk1 and Cdk2 inhibitors as a general approach in cancer therapy.

Detaljer

Författare
  • Lakshmi Gopinathan
  • Shawn Lu Wen Tan
  • V. C. Padmakumar
  • Vincenzo Coppola
  • Lino Tessarollo
  • Philipp Kaldis
Externa organisationer
  • Institute of Molecular and Cell Biology
  • University of Cambridge
  • National Cancer Institute at Frederick
  • National University of Singapore
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
Originalspråkengelska
Sidor (från-till)3870-3879
Antal sidor10
TidskriftCancer Research
Volym74
Utgivningsnummer14
StatusPublished - 2014 jul
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa