Lung Inflammatory Environments Differentially Alter Mesenchymal Stromal Cell Behavior

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Mesenchymal stromal (stem) cells (MSCs) are increasingly demonstrated to ameliorate experimentally-induced lung injuries through disease-specific anti-inflammatory actions, thus suggesting that different in vivo inflammatory environments can influence MSC actions. To determine the effects of different representative inflammatory lung conditions, human bone marrow-derived MSCs (hMSCs) were exposed to in vitro culture conditions from bronchoalveolar lavage fluid (BALF) samples obtained from patients with either the acute respiratory distress syndrome (ARDS) or with other lung diseases including acute respiratory exacerbations of cystic fibrosis (CF) (non-ARDS). hMSCs were subsequently assessed for time- and BALF concentration-dependent effects on mRNA expression of selected pro- and anti-inflammatory mediators, and for overall patterns of gene and mRNA expression. Both common and disease specific-patterns were observed in gene expression of different hMSC mediators, notably interleukin (IL)-6. Conditioned media obtained from non-ARDS BALF-exposed hMSCs was more effective in promoting an anti-inflammatory phenotype in monocytes than was conditioned media from ARDS BALF-exposed hMSCs. Neutralizing IL-6 in the conditioned media promoted generation of anti-inflammatory monocyte phenotype. These results demonstrated that different lung inflammatory environments differentially alter hMSC behavior. Further identification of these interactions and the driving mechanisms may influence clinical use of MSCs for treating lung diseases.

Detaljer

Författare
  • Soraia Carvalho Abreu
  • Sara Rolandsson Enes
  • Jacob Dearborn
  • Meagan Goodwin
  • Amy Coffey
  • Zachary D Borg
  • Claudia C Dos Santos
  • Matthew J Wargo
  • Fernanda Ferreira Cruz
  • Roberto Loi
  • Michael DeSarno
  • Takamaru Ashikaga
  • Mariana Alves Antunes
  • Patricia R M Rocco
  • Kathleen D Liu
  • Jae-Woo Lee
  • Michael A Matthay
  • D H McKenna
  • Daniel J Weiss
Enheter & grupper
Externa organisationer
  • University of Vermont
  • University of Toronto
  • University of California, San Francisco
  • Federal University of Rio de Janeiro
  • University of Cagliari
  • University of Minnesota system
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Mikrobiologi inom det medicinska området
Originalspråkengelska
Sidor (från-till)L823-L831
Antal sidor9
TidskriftAmerican Journal of Physiology: Lung Cellular and Molecular Physiology
Volym317
Utgåva nummer6
Tidigt onlinedatum2019 sep 25
StatusPublished - 2019 dec 1
PublikationskategoriForskning
Peer review utfördJa