Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10–100 μm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 μm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1–10 μm in size) or even whole blood containing blood cells (up to 20 μm in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent.

Detaljer

Författare
  • W. Noppe
  • Fatima Plieva
  • K. Vanhoorelbeke
  • H. Deckmyn
  • M. Tuncel
  • A. Tuncel
  • Igor Galaev
  • Bo Mattiasson
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Industriell bioteknik

Nyckelord

Originalspråkengelska
Sidor (från-till)293-299
TidskriftJournal of Biotechnology
Volym131
Utgivningsnummer3
StatusPublished - 2007
PublikationskategoriForskning
Peer review utfördJa