Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres. / Mohlin, Sofie; Kunttas, Ezgi; Persson, Camilla U.; Abdel-Haq, Reem; Castillo, Aldo; Murko, Christina; Bronner, Marianne E.; Kerosuo, Laura.

I: Developmental Biology, Vol. 447, Nr. 2, 2019, s. 137-146.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Mohlin, S, Kunttas, E, Persson, CU, Abdel-Haq, R, Castillo, A, Murko, C, Bronner, ME & Kerosuo, L 2019, 'Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres', Developmental Biology, vol. 447, nr. 2, s. 137-146. https://doi.org/10.1016/j.ydbio.2019.01.010

APA

Mohlin, S., Kunttas, E., Persson, C. U., Abdel-Haq, R., Castillo, A., Murko, C., ... Kerosuo, L. (2019). Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres. Developmental Biology, 447(2), 137-146. https://doi.org/10.1016/j.ydbio.2019.01.010

CBE

Mohlin S, Kunttas E, Persson CU, Abdel-Haq R, Castillo A, Murko C, Bronner ME, Kerosuo L. 2019. Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres. Developmental Biology. 447(2):137-146. https://doi.org/10.1016/j.ydbio.2019.01.010

MLA

Vancouver

Author

Mohlin, Sofie ; Kunttas, Ezgi ; Persson, Camilla U. ; Abdel-Haq, Reem ; Castillo, Aldo ; Murko, Christina ; Bronner, Marianne E. ; Kerosuo, Laura. / Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres. I: Developmental Biology. 2019 ; Vol. 447, Nr. 2. s. 137-146.

RIS

TY - JOUR

T1 - Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres

AU - Mohlin, Sofie

AU - Kunttas, Ezgi

AU - Persson, Camilla U.

AU - Abdel-Haq, Reem

AU - Castillo, Aldo

AU - Murko, Christina

AU - Bronner, Marianne E.

AU - Kerosuo, Laura

PY - 2019

Y1 - 2019

N2 - Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest stemness and lineage decisions as well as accompanying diseases.

AB - Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest stemness and lineage decisions as well as accompanying diseases.

KW - Crestospheres

KW - Multipotency, self-renewal

KW - Neural crest stem cells

KW - Stem cell maintenance

KW - Trunk neural crest

U2 - 10.1016/j.ydbio.2019.01.010

DO - 10.1016/j.ydbio.2019.01.010

M3 - Article

VL - 447

SP - 137

EP - 146

JO - Developmental Biology

T2 - Developmental Biology

JF - Developmental Biology

SN - 1095-564X

IS - 2

ER -