Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II
Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift
In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.
Ämnesklassifikation (UKÄ) – OBLIGATORISK
|Tidskrift||Journal of Cell Biology|
|Status||Published - 2014 sep 29|
|Peer review utförd||Ja|