Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.

Detaljer

Författare
  • Deepak Adhikari
  • M. Kasim Diril
  • Kiran Busayavalasa
  • Sanjiv Risal
  • Shoma Nakagawa
  • Rebecca Lindkvist
  • Yan Shen
  • Vincenzo Coppola
  • Lino Tessarollo
  • Nobuaki R. Kudo
  • Philipp Kaldis
  • Kui Liu
Externa organisationer
  • Göteborgs universitet
  • Institute of Molecular and Cell Biology
  • Hammersmith Hospital
  • National Cancer Institute at Frederick
  • National University of Singapore
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
Originalspråkengelska
Sidor (från-till)843-853
TidskriftJournal of Cell Biology
Volym206
Utgåva nummer7
StatusPublished - 2014 sep 29
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa