Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II

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Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II. / Adhikari, Deepak; Diril, M. Kasim; Busayavalasa, Kiran; Risal, Sanjiv; Nakagawa, Shoma; Lindkvist, Rebecca; Shen, Yan; Coppola, Vincenzo; Tessarollo, Lino; Kudo, Nobuaki R.; Kaldis, Philipp; Liu, Kui.

I: Journal of Cell Biology, Vol. 206, Nr. 7, 29.09.2014, s. 843-853.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Adhikari, D, Diril, MK, Busayavalasa, K, Risal, S, Nakagawa, S, Lindkvist, R, Shen, Y, Coppola, V, Tessarollo, L, Kudo, NR, Kaldis, P & Liu, K 2014, 'Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II', Journal of Cell Biology, vol. 206, nr. 7, s. 843-853. https://doi.org/10.1083/jcb.201406033

APA

Adhikari, D., Diril, M. K., Busayavalasa, K., Risal, S., Nakagawa, S., Lindkvist, R., Shen, Y., Coppola, V., Tessarollo, L., Kudo, N. R., Kaldis, P., & Liu, K. (2014). Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II. Journal of Cell Biology, 206(7), 843-853. https://doi.org/10.1083/jcb.201406033

CBE

Adhikari D, Diril MK, Busayavalasa K, Risal S, Nakagawa S, Lindkvist R, Shen Y, Coppola V, Tessarollo L, Kudo NR, Kaldis P, Liu K. 2014. Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II. Journal of Cell Biology. 206(7):843-853. https://doi.org/10.1083/jcb.201406033

MLA

Vancouver

Adhikari D, Diril MK, Busayavalasa K, Risal S, Nakagawa S, Lindkvist R et al. Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II. Journal of Cell Biology. 2014 sep 29;206(7):843-853. https://doi.org/10.1083/jcb.201406033

Author

Adhikari, Deepak ; Diril, M. Kasim ; Busayavalasa, Kiran ; Risal, Sanjiv ; Nakagawa, Shoma ; Lindkvist, Rebecca ; Shen, Yan ; Coppola, Vincenzo ; Tessarollo, Lino ; Kudo, Nobuaki R. ; Kaldis, Philipp ; Liu, Kui. / Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II. I: Journal of Cell Biology. 2014 ; Vol. 206, Nr. 7. s. 843-853.

RIS

TY - JOUR

T1 - Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II

AU - Adhikari, Deepak

AU - Diril, M. Kasim

AU - Busayavalasa, Kiran

AU - Risal, Sanjiv

AU - Nakagawa, Shoma

AU - Lindkvist, Rebecca

AU - Shen, Yan

AU - Coppola, Vincenzo

AU - Tessarollo, Lino

AU - Kudo, Nobuaki R.

AU - Kaldis, Philipp

AU - Liu, Kui

PY - 2014/9/29

Y1 - 2014/9/29

N2 - In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.

AB - In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.

UR - http://www.scopus.com/inward/record.url?scp=84907764457&partnerID=8YFLogxK

U2 - 10.1083/jcb.201406033

DO - 10.1083/jcb.201406033

M3 - Article

C2 - 25246615

AN - SCOPUS:84907764457

VL - 206

SP - 843

EP - 853

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 7

ER -