Mathematical modelling and parametric studies of affinity chromatography

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Mathematical modelling and parametric studies of affinity chromatography. / Carlsson, F; Axelsson, Anders; Zacchi, Guido.

I: Computers & Chemical Engineering, Vol. 18, Nr. suppl. 1, 1994, s. S657-S661.

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TY - JOUR

T1 - Mathematical modelling and parametric studies of affinity chromatography

AU - Carlsson, F

AU - Axelsson, Anders

AU - Zacchi, Guido

PY - 1994

Y1 - 1994

N2 - In this study a model based on mass transfer and sorption rate constants with physical significance is used for simulation of affinity chromatography, The simulation program has been used to perform a parametric analysis of the adsorption of lysozyme on Cibacron Blue-Sepharose CL-6B. The influence of process parameters as well as physical parameters on the chromatography process was investigated. The external and the internal mass transfer as well as the sorption rate were found to contribute to the control of the overall rate of the system. The most important process parameter was the radius of the beads. The optimum bead size was found to be around 50 mu m.

AB - In this study a model based on mass transfer and sorption rate constants with physical significance is used for simulation of affinity chromatography, The simulation program has been used to perform a parametric analysis of the adsorption of lysozyme on Cibacron Blue-Sepharose CL-6B. The influence of process parameters as well as physical parameters on the chromatography process was investigated. The external and the internal mass transfer as well as the sorption rate were found to contribute to the control of the overall rate of the system. The most important process parameter was the radius of the beads. The optimum bead size was found to be around 50 mu m.

KW - AFFINITY CHROMATOGRAPHY

KW - ION EXCHANGE-CHROMATOGRAPHY

KW - SIMULATION

KW - MODELING

KW - LYSOZYME

U2 - 10.1016/0098-1354(94)80107-X

DO - 10.1016/0098-1354(94)80107-X

M3 - Article

VL - 18

SP - S657-S661

JO - Computers and Chemical Engineering

JF - Computers and Chemical Engineering

SN - 1873-4375

IS - suppl. 1

ER -