MECHANISMS OF THE BENEFICIAL EFFECT OF HYPERTONIC SALINE SOLUTION IN ACUTE PANCREATITIS.

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MECHANISMS OF THE BENEFICIAL EFFECT OF HYPERTONIC SALINE SOLUTION IN ACUTE PANCREATITIS. / Coelho, Ana Maria Mendonça; Jukemura, José; Sampietre, Sandra N; Martins, Joilson O; Molan, Nilza A T; Patzina, Rosely A; Lindkvist, Björn; Jancar, Sonia; Cunha, José Eduardo M; D'Albuquerque, Luiz A Carneiro; Machado, Marcel Cerqueira Cesar.

I: Shock, Vol. 34, 2010, s. 502-507.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Coelho, AMM, Jukemura, J, Sampietre, SN, Martins, JO, Molan, NAT, Patzina, RA, Lindkvist, B, Jancar, S, Cunha, JEM, D'Albuquerque, LAC & Machado, MCC 2010, 'MECHANISMS OF THE BENEFICIAL EFFECT OF HYPERTONIC SALINE SOLUTION IN ACUTE PANCREATITIS.', Shock, vol. 34, s. 502-507. https://doi.org/10.1097/SHK.0b013e3181defaa1

APA

Coelho, A. M. M., Jukemura, J., Sampietre, S. N., Martins, J. O., Molan, N. A. T., Patzina, R. A., Lindkvist, B., Jancar, S., Cunha, J. E. M., D'Albuquerque, L. A. C., & Machado, M. C. C. (2010). MECHANISMS OF THE BENEFICIAL EFFECT OF HYPERTONIC SALINE SOLUTION IN ACUTE PANCREATITIS. Shock, 34, 502-507. https://doi.org/10.1097/SHK.0b013e3181defaa1

CBE

Coelho AMM, Jukemura J, Sampietre SN, Martins JO, Molan NAT, Patzina RA, Lindkvist B, Jancar S, Cunha JEM, D'Albuquerque LAC, Machado MCC. 2010. MECHANISMS OF THE BENEFICIAL EFFECT OF HYPERTONIC SALINE SOLUTION IN ACUTE PANCREATITIS. Shock. 34:502-507. https://doi.org/10.1097/SHK.0b013e3181defaa1

MLA

Vancouver

Coelho AMM, Jukemura J, Sampietre SN, Martins JO, Molan NAT, Patzina RA et al. MECHANISMS OF THE BENEFICIAL EFFECT OF HYPERTONIC SALINE SOLUTION IN ACUTE PANCREATITIS. Shock. 2010;34:502-507. https://doi.org/10.1097/SHK.0b013e3181defaa1

Author

Coelho, Ana Maria Mendonça ; Jukemura, José ; Sampietre, Sandra N ; Martins, Joilson O ; Molan, Nilza A T ; Patzina, Rosely A ; Lindkvist, Björn ; Jancar, Sonia ; Cunha, José Eduardo M ; D'Albuquerque, Luiz A Carneiro ; Machado, Marcel Cerqueira Cesar. / MECHANISMS OF THE BENEFICIAL EFFECT OF HYPERTONIC SALINE SOLUTION IN ACUTE PANCREATITIS. I: Shock. 2010 ; Vol. 34. s. 502-507.

RIS

TY - JOUR

T1 - MECHANISMS OF THE BENEFICIAL EFFECT OF HYPERTONIC SALINE SOLUTION IN ACUTE PANCREATITIS.

AU - Coelho, Ana Maria Mendonça

AU - Jukemura, José

AU - Sampietre, Sandra N

AU - Martins, Joilson O

AU - Molan, Nilza A T

AU - Patzina, Rosely A

AU - Lindkvist, Björn

AU - Jancar, Sonia

AU - Cunha, José Eduardo M

AU - D'Albuquerque, Luiz A Carneiro

AU - Machado, Marcel Cerqueira Cesar

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Emergency medicine/Medicine/Surgery (013240200)

PY - 2010

Y1 - 2010

N2 - BACKGROUND/AIMS:: Administration of hypertonic saline (HS) solution to rats with acute pancreatitis (AP) decreases mortality and systemic inflammation. We hypothesized that these effects are relates not only to systemic inflammatory reduction but also to reduction of the pancreatic lesion. METHODS:: AP was induced in Wistar rats by injection of 2.5% sodium taurocholate. Animals were divided in groups: C (without AP), NT (not treated AP), NS (AP treated with NaCl 0.9%), and HS (AP treated with NaCl 7.5%). RESULTS:: Trypsinogen activation peptides (TAP) and amylase activity were increased in ascitic fluid and serum and were not affected by treatment with HS. Pancreas inflammation was evaluated by increased myeloperoxidase (MPO) activity, malonyldialdehyde (MDA) formation and histopathology for severity of pancreatic lesions. HS did not affect these parameters. Expression of cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) was markedly increased in the pancreas of the AP group and was reduced by treatment with HS. This treatment also reduced the levels of TNF-alpha and IL-6 but not of IL-10 in the pancreatic tissue. CONCLUSIONS:: These results show that HS modulates cytokines production and expression of enzymes responsible for inflammatory mediators production in the pancreas without affecting the severity of the pancreatic lesions.

AB - BACKGROUND/AIMS:: Administration of hypertonic saline (HS) solution to rats with acute pancreatitis (AP) decreases mortality and systemic inflammation. We hypothesized that these effects are relates not only to systemic inflammatory reduction but also to reduction of the pancreatic lesion. METHODS:: AP was induced in Wistar rats by injection of 2.5% sodium taurocholate. Animals were divided in groups: C (without AP), NT (not treated AP), NS (AP treated with NaCl 0.9%), and HS (AP treated with NaCl 7.5%). RESULTS:: Trypsinogen activation peptides (TAP) and amylase activity were increased in ascitic fluid and serum and were not affected by treatment with HS. Pancreas inflammation was evaluated by increased myeloperoxidase (MPO) activity, malonyldialdehyde (MDA) formation and histopathology for severity of pancreatic lesions. HS did not affect these parameters. Expression of cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) was markedly increased in the pancreas of the AP group and was reduced by treatment with HS. This treatment also reduced the levels of TNF-alpha and IL-6 but not of IL-10 in the pancreatic tissue. CONCLUSIONS:: These results show that HS modulates cytokines production and expression of enzymes responsible for inflammatory mediators production in the pancreas without affecting the severity of the pancreatic lesions.

U2 - 10.1097/SHK.0b013e3181defaa1

DO - 10.1097/SHK.0b013e3181defaa1

M3 - Article

C2 - 20351627

VL - 34

SP - 502

EP - 507

JO - Shock

JF - Shock

SN - 1540-0514

ER -