Methods for the determination in the nanomole range of lipids in liver fine-needle aspiration biopsies

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Triglycerides and phospholipids 0.5-2mg (wet weight) of human liver material obtained by the fine-needle aspiration biopsy technique can be estimated after chloroform:methanol extraction by the determination of lipid phosphorus and glyceride glycerol. The methods are based on the colorimetric determination of inorganic phosphate after oxidation of the phospholipids and the enzymatic fluorometric determination of glycerol after complete hydrolysis of the triglycerides, catalyzed by purified pancreatic lipase. The accumulation of lipids is then expressed as the increase in the molar ratio of triglycerides over phospholipids.

Detaljer

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Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Pediatrik

Nyckelord

Originalspråkengelska
Sidor (från-till)53-60
TidskriftScandinavian Journal of Clinical & Laboratory Investigation
Volym26
Utgivningsnummer1
StatusPublished - 1970
PublikationskategoriForskning
Peer review utfördJa