Methods for the preparation of chlorophyllide a: an intermediate of the chlorophyll biosynthetic pathway

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid phase extraction, yielding 14 mug of chlorophyllide a per gram of barley leaves. Chlorophyllide a was identified by thin layer chromatography, absorption spectroscopy, and mass spectrometry.

Detaljer

Författare
  • A. H. Muller
  • S. P. Gough
  • D. W. Bollivar
  • M. Meldal
  • R. D. Willows
  • Mats Hansson
Externa organisationer
  • Carlsberg Research Center / Carlsberg Laboratory
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokemi och molekylärbiologi

Nyckelord

Originalspråkengelska
Sidor (från-till)271-276
TidskriftAnalytical Biochemistry
Volym419
Utgåva nummer2
StatusPublished - 2011
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa