Minimal exclusion of plasma membrane proteins during retroviral envelope formation

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The retrovirus forms its envelope by budding at the plasma membrane (PM). This process is primarily driven by its cytoplasmic core-precursor protein, Gag, as shown by the efficient formation of virus-like Gag particles in the absence of its envelope protein, Env. Most interestingly, several studies have demonstrated incorporation of various PM proteins into retrovirus, but the underlying mechanism of this phenomenon has remained elusive. We have purified Moloney murine leukemia virus Gag particles by sedimentation in an iodixanol gradient and donor PMs by flotation in a sucrose gradient and compared their protein compositions at equal lipid basis. We found that most PM proteins are present at similar density in both membranes. The inclusion of PM proteins was unaffected by incorporation of Env protein into the envelope of the Gag particles and whether these were produced at high or low level in the cells. These findings indicate that most PM proteins become incorporated into the retrovirus envelope without significant sorting. This feature of retrovirus assembly should be considered when studying retrovirus functions and developing retrovirus vectors.

Detaljer

Författare
  • Maria Hammarstedt
  • K Wallengren
  • K W Pedersen
  • N Roos
  • H Garoff
Externa organisationer
  • External Organization - Unknown
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
Originalspråkengelska
Sidor (från-till)7527-7532
TidskriftProceedings of the National Academy of Sciences
Volym97
Utgivningsnummer13
StatusPublished - 2000
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa