Mitochondrial Respiratory Function in Peripheral Blood Cells from Huntington's Disease Patients

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Background

Patients with Huntington's disease display symptoms from both the central nervous system and peripheral tissues. Mitochondrial dysfunction has been implicated as part of the pathogenesis of the disease and has been reported in brain tissue and extracerebral tissues, such as muscle and blood cells, but the results are inconsistent. Therefore, the authors performed a refined evaluation of mitochondrial function in 2 types of peripheral blood cells from 14 patients with Huntington's disease and 21 control subjects. Several hypotheses were predefined, including impaired mitochondrial complex II function (primary), complex I function (secondary), and maximum oxidative phosphorylation capacity (secondary) in patient cells.

Methods

High-resolution respirometry was applied to viable platelets and mononuclear cells. Data were normalized to cell counts, citrate synthase activity, and mitochondrial DNA copy numbers.

Results

Normalized to citrate synthase activity, platelets from patients with Huntington's disease displayed respiratory dysfunction linked to complex I, complex II, and lower maximum oxidative phosphorylation capacity. No difference was seen in mononuclear cells or when platelet data were normalized to cell counts or mitochondrial DNA. The ratio of complex I respiration through maximum oxidative phosphorylation was significantly decreased in patients compared with controls. The corresponding ratio for complex II was unaffected.

Conclusions

The data indicate decreased function of mitochondrial complex I in peripheral blood cells from patients with Huntington's disease, although this could not be uniformly confirmed. The results do not confirm a systemic complex II dysfunction and do not currently support the use of mitochondrial function in blood cells as a biomarker for the disease.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Skåne University Hospital
  • National Center for Child Health and Development
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Neurologi

Nyckelord

Originalspråkengelska
Sidor (från-till)472–482
TidskriftMovement Disorders Clinical Practice
Volym3
Utgåva nummer5
StatusPublished - 2016 sep
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Johannes Ehinger, 2017, Lund: Lund University: Faculty of Medicine. 75 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

Johannes Ehinger, Saori Morota, Magnus Hansson, Gesine Paul-Visse & Eskil Elmer, 2015, I: Journal of Neurology. 262, 6, s. 1493-1503

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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