Mobility of Core Water in Bacillus subtilis Spores by (2)H NMR.

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Mobility of Core Water in Bacillus subtilis Spores by (2)H NMR. / Kaieda, Shuji; Setlow, Barbara; Setlow, Peter; Halle, Bertil.

I: Biophysical Journal, Vol. 105, Nr. 9, 2013, s. 2016-2023.

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Kaieda, Shuji ; Setlow, Barbara ; Setlow, Peter ; Halle, Bertil. / Mobility of Core Water in Bacillus subtilis Spores by (2)H NMR. I: Biophysical Journal. 2013 ; Vol. 105, Nr. 9. s. 2016-2023.

RIS

TY - JOUR

T1 - Mobility of Core Water in Bacillus subtilis Spores by (2)H NMR.

AU - Kaieda, Shuji

AU - Setlow, Barbara

AU - Setlow, Peter

AU - Halle, Bertil

PY - 2013

Y1 - 2013

N2 - Bacterial spores in a metabolically dormant state can survive long periods without nutrients under extreme environmental conditions. The molecular basis of spore dormancy is not well understood, but the distribution and physical state of water within the spore is thought to play an important role. Two scenarios have been proposed for the spore's core region, containing the DNA and most enzymes. In the gel scenario, the core is a structured macromolecular framework permeated by mobile water. In the glass scenario, the entire core, including the water, is an amorphous solid and the quenched molecular diffusion accounts for the spore's dormancy and thermal stability. Here, we use (2)H magnetic relaxation dispersion to selectively monitor water mobility in the core of Bacillus subtilis spores in the presence and absence of core Mn(2+) ions. We also report and analyze the solid-state (2)H NMR spectrum from these spores. Our NMR data clearly support the gel scenario with highly mobile core water (∼25 ps average rotational correlation time). Furthermore, we find that the large depot of manganese in the core is nearly anhydrous, with merely 1.7% on average of the maximum sixfold water coordination.

AB - Bacterial spores in a metabolically dormant state can survive long periods without nutrients under extreme environmental conditions. The molecular basis of spore dormancy is not well understood, but the distribution and physical state of water within the spore is thought to play an important role. Two scenarios have been proposed for the spore's core region, containing the DNA and most enzymes. In the gel scenario, the core is a structured macromolecular framework permeated by mobile water. In the glass scenario, the entire core, including the water, is an amorphous solid and the quenched molecular diffusion accounts for the spore's dormancy and thermal stability. Here, we use (2)H magnetic relaxation dispersion to selectively monitor water mobility in the core of Bacillus subtilis spores in the presence and absence of core Mn(2+) ions. We also report and analyze the solid-state (2)H NMR spectrum from these spores. Our NMR data clearly support the gel scenario with highly mobile core water (∼25 ps average rotational correlation time). Furthermore, we find that the large depot of manganese in the core is nearly anhydrous, with merely 1.7% on average of the maximum sixfold water coordination.

U2 - 10.1016/j.bpj.2013.09.022

DO - 10.1016/j.bpj.2013.09.022

M3 - Article

VL - 105

SP - 2016

EP - 2023

JO - Biophysical Journal

JF - Biophysical Journal

SN - 1542-0086

IS - 9

ER -