Modeling the evolution of culture-adapted human embryonic stem cells

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The long-term culture of human embryonic stem (ES) cells is inevitably subject to evolution, since any mutant that arises with a growth advantage will be selectively amplified. However, the evolutionary influences of population size, mutation rate, and selection pressure are frequently overlooked. We have constructed a Monte Carlo simulation model to predict how changes in these factors can influence the appearance and spread of mutant ES cells, and verified its applicability by comparison with in vitro data. This simulation provides an estimate for the expected rate of generation of culture-adapted ES cells under different assumptions for the key parameters. In particular, it highlights the effect of population size, suggesting that the maintenance of cells in small populations reduces the likelihood that abnormal cultures will develop.

Detaljer

Författare
  • Victor Olariu
  • Neil J. Harrison
  • Daniel Coca
  • Paul J. Gokhale
  • Duncan Baker
  • Steve Billings
  • Visakan Kadirkamanathan
  • Peter W. Andrews
Externa organisationer
  • University of Sheffield
  • Sheffield Children's Trust
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
  • Biofysik
Originalspråkengelska
Sidor (från-till)50-56
Antal sidor7
TidskriftStem Cell Research
Volym4
Utgåva nummer1
StatusPublished - 2010 jan
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa