Modeling the evolution of culture-adapted human embryonic stem cells

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Modeling the evolution of culture-adapted human embryonic stem cells. / Olariu, Victor; Harrison, Neil J.; Coca, Daniel; Gokhale, Paul J.; Baker, Duncan; Billings, Steve; Kadirkamanathan, Visakan; Andrews, Peter W.

I: Stem Cell Research, Vol. 4, Nr. 1, 01.2010, s. 50-56.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Olariu, V, Harrison, NJ, Coca, D, Gokhale, PJ, Baker, D, Billings, S, Kadirkamanathan, V & Andrews, PW 2010, 'Modeling the evolution of culture-adapted human embryonic stem cells', Stem Cell Research, vol. 4, nr. 1, s. 50-56. https://doi.org/10.1016/j.scr.2009.09.001

APA

Olariu, V., Harrison, N. J., Coca, D., Gokhale, P. J., Baker, D., Billings, S., ... Andrews, P. W. (2010). Modeling the evolution of culture-adapted human embryonic stem cells. Stem Cell Research, 4(1), 50-56. https://doi.org/10.1016/j.scr.2009.09.001

CBE

Olariu V, Harrison NJ, Coca D, Gokhale PJ, Baker D, Billings S, Kadirkamanathan V, Andrews PW. 2010. Modeling the evolution of culture-adapted human embryonic stem cells. Stem Cell Research. 4(1):50-56. https://doi.org/10.1016/j.scr.2009.09.001

MLA

Vancouver

Author

Olariu, Victor ; Harrison, Neil J. ; Coca, Daniel ; Gokhale, Paul J. ; Baker, Duncan ; Billings, Steve ; Kadirkamanathan, Visakan ; Andrews, Peter W. / Modeling the evolution of culture-adapted human embryonic stem cells. I: Stem Cell Research. 2010 ; Vol. 4, Nr. 1. s. 50-56.

RIS

TY - JOUR

T1 - Modeling the evolution of culture-adapted human embryonic stem cells

AU - Olariu, Victor

AU - Harrison, Neil J.

AU - Coca, Daniel

AU - Gokhale, Paul J.

AU - Baker, Duncan

AU - Billings, Steve

AU - Kadirkamanathan, Visakan

AU - Andrews, Peter W.

PY - 2010/1

Y1 - 2010/1

N2 - The long-term culture of human embryonic stem (ES) cells is inevitably subject to evolution, since any mutant that arises with a growth advantage will be selectively amplified. However, the evolutionary influences of population size, mutation rate, and selection pressure are frequently overlooked. We have constructed a Monte Carlo simulation model to predict how changes in these factors can influence the appearance and spread of mutant ES cells, and verified its applicability by comparison with in vitro data. This simulation provides an estimate for the expected rate of generation of culture-adapted ES cells under different assumptions for the key parameters. In particular, it highlights the effect of population size, suggesting that the maintenance of cells in small populations reduces the likelihood that abnormal cultures will develop.

AB - The long-term culture of human embryonic stem (ES) cells is inevitably subject to evolution, since any mutant that arises with a growth advantage will be selectively amplified. However, the evolutionary influences of population size, mutation rate, and selection pressure are frequently overlooked. We have constructed a Monte Carlo simulation model to predict how changes in these factors can influence the appearance and spread of mutant ES cells, and verified its applicability by comparison with in vitro data. This simulation provides an estimate for the expected rate of generation of culture-adapted ES cells under different assumptions for the key parameters. In particular, it highlights the effect of population size, suggesting that the maintenance of cells in small populations reduces the likelihood that abnormal cultures will develop.

UR - http://www.scopus.com/inward/record.url?scp=73149094719&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2009.09.001

DO - 10.1016/j.scr.2009.09.001

M3 - Article

VL - 4

SP - 50

EP - 56

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1876-7753

IS - 1

ER -