Modulation of epileptogenesis: A paradigm for the integration of enzyme-based microelectrode arrays and optogenetics

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Background
Genesis of acquired epilepsy includes transformations spanning genetic-to- network-level modifications, disrupting the regional excitatory/inhibitory balance. Methodology concurrently tracking changes at multiple levels is lacking. Here, viral vectors are used to differentially express two opsin proteins in neuronal populations within dentate gyrus (DG) of hippocampus. When activated, these opsins induced excitatory or inhibitory neural output that differentially affected neural networks and epileptogenesis. In vivo measures included behavioral observation coupled to real-time measures of regional glutamate flux using ceramic-based amperometric microelectrode arrays (MEAs).

Detaljer

Författare
  • Corwin R. Butler
  • Jeffery A. Boychuk
  • Francois Pomerleau
  • Ramona Alcala
  • Peter Huettl
  • Yi Ai
  • Johan Jakobsson
  • Sidney W. Whiteheart
  • Greg A. Gerhardt
  • Bret N. Smith
  • John T Slevin
Enheter & grupper
Externa organisationer
  • University of Kentucky
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Neurovetenskaper
Originalspråkengelska
Artikelnummer106244
TidskriftEpilepsy Research
Volym159
StatusPublished - 2020 jan 20
PublikationskategoriForskning
Peer review utfördJa