Molecular basis of commensalism in the urinary tract: low virulence or virulence attenuation?

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In some patients Escherichia coli strains establish significant bacteriuria without causing symptoms of urinary tract infection (UTI). These asymptomatic bacteriuria (ABU) strains have been shown to express fewer virulence factors than the uropathogenic E. coli (UPEC) strains that cause severe, symptomatic UTI. Paradoxically, ABU strains carry many typical UPEC virulence genes, however, and the molecular basis of their low virulence therefore remains unclear. This study examined if ABU strains may evolve from UPEC by genome loss and virulence gene attenuation. The presence of conserved E. coli K-12 genes was examined using an E. coli K-12 strain MG1655-specific DNA array and the distribution of UPEC virulence-related genes with the E. coli pathoarray. Two groups of strains could be distinguished. Several ABU strains were shown by multi locus sequence typing and by comparative genomic analyses to be related to UPEC but to have smaller genome sizes. There were significant alterations in essential virulence genes, including reductive evolution by point mutations, DNA rearrangements and deletions. Other strains were unrelated to UPEC and lacked most of the virulence-associated genes. The results suggest that some ABU strains arise from virulent strains by attenuation of virulence genes while others are non-virulent and resemble commensal strains. We propose that virulence attenuation might constitute a general mechanism for mucosal pathogens to evolve towards commensalism.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Immunologi inom det medicinska området
  • Mikrobiologi inom det medicinska området
  • Urologi och njurmedicin
Originalspråkengelska
Sidor (från-till)695-703
TidskriftInfection and Immunity
Volym76
Utgivningsnummer2
StatusPublished - 2008
PublikationskategoriForskning
Peer review utfördJa