Molecular mechanisms controlling phosphate-induced downregulation of the yeast Pho84 phosphate transporter

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In Saccharomyces cerevisiae, phosphate uptake is mainly dependent on the proton-coupled Pho84 permease under phosphate-limited growth conditions. Phosphate addition causes Pho84-mediated activation of the protein kinase A (PKA) pathway as well as rapid internalization and vacuolar breakdown of Pho84. We show that Pho84 undergoes phosphate-induced phosphorylation and subsequent ubiquitination on amino acids located in the large middle intracellular loop prior to endocytosis. The attachment of ubiquitin is dependent on the ubiquitin conjugating enzymes Ubc2 and Ubc4. In addition, we show that the Pho84 endocytotic process is delayed in strains with reduced PKA activity. Our results suggest that Pho84-mediated activation of the PKA pathway is responsible for its own downregulation by phosphorylation, ubiquination, internalization, and vacuolar breakdown.

Detaljer

Författare
  • Fredrik Lundh
  • Jean-Marie Mouillon
  • Dieter Samyn
  • Kent Stadler
  • Yulia Popova
  • Jens O Lagerstedt
  • Johan M Thevelein
  • Bengt L Persson
Externa organisationer
  • University of Kalmar
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)4497-505
Antal sidor9
TidskriftBiochemistry
Volym48
Utgivningsnummer21
StatusPublished - 2009 jun 2
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa