Monitoring of Saccharomyces cerevisiae Cell Proliferation on Thiol-Modified Planar Gold Microelectrodes Using Impedance Spectroscopy.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

An impedance spectroscopic study of the interaction between thiol-modified Au electrodes and Saccharomyces cerevisiae of strain EBY44 revealed that the cells formed an integral part of the interface, modulating the capacitive properties until a complete monolayer was obtained, whereas the charge transfer resistance ( R ct) to the redox process of [Fe(CN)6] (3-/4-) showed a linear relationship to the number of cells even beyond the monolayer coverage. R ct showed strong pH dependence upon increasing the pH of the utilized buffer to 7.2. Upon addition of S. cerevisiae cells at pH 7.2, the obtained value of R ct showed over 560% increase with respect to the value obtained on the same thiol-modified electrode without cells. It was demonstrated that real-time monitoring of S. cerevisiae proliferation, with frequency-normalized imaginary admittance (real capacitance) as the indicator, was possible using a miniaturized culture system, ECIS Cultureware, with integrated planar cysteamine-modified Au microelectrodes. A monolayer coverage was reached after 20-28 h of cultivation, observed as an approximately 15% decrease in the real capacitance of the system.

Detaljer

Författare
  • Arto Heiskanen
  • Christer Spegel
  • Natalie Kostesha
  • Tautgirdas Ruzgas
  • Jenny Emnéus
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Industriell bioteknik
  • Analytisk kemi
Originalspråkengelska
Sidor (från-till)9066-9073
TidskriftLangmuir
Volym24
Utgåva nummer16
StatusPublished - 2008
PublikationskategoriForskning
Peer review utfördJa