Multiplexed MALDI-MS arrays for screening of MIP solid phase extraction materials.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Technology that facilitates rapid investigation of solid phase extraction protocols using very small amounts of sorbent can save both time and money. The microfabricated ISET (Integrated Selective Enrichment Target) interfaced with MALDI mass spectrometry is able to provide an efficient, economic and generic optimization process for SPE sample preparation. The SPE is performed in a rapid and parallel fashion, with a processing time off only 2h per ISET with 96 samples. Each of the 96 wells on the ISET can hold 600nL of SPE sorbent. The ability to work with small amounts of sorbent and samples in the ISET platform provides a big advantage when developing affinity sorbents, such as molecularly imprinted polymers (MIPs). Here it is demonstrated that an amount of 25mg phosphoserine imprinted MIP (pS-MIP) sorbent can allow for analysis of more than 500 ISET nanovials using a multitude of different conditions. In the presented case, the multiplexed experiments allowed for early discovery of unspecific interactions and subsequent minimization of these, resulting in a protocol that provided improved enrichment of phosphopeptides.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biomedicinsk laboratorievetenskap/teknologi
Originalspråkengelska
Sidor (från-till)213-220
TidskriftJournal of Chromatography. B
Volym1021
Tidigt onlinedatum2015 okt 30
StatusPublished - 2016
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Kishore Jagadeesan, Cecilia Rossetti, Abed Abdel Qader, Léon Reubsaet, Borje Sellergren, Thomas Laurell & Simon Ekström, 2017 dec 1, I : SLAS Discovery. 22, 10, s. 1253-1261

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Kishore Jagadeesan, 2017 apr 28, Tryckeriet E-huset, Lund: Department of Biomedical Engineering, Lund university. 135 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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Related projects

Kishore Kumar Jagadeesan, Simon Ekström & Thomas Laurell

2012/01/012015/12/31

Projekt: NätverkInternationellt samarbete

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