Multiplexing of miniaturized planar antibody arrays for serum protein profiling - a biomarker discovery in SLE nephritis.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In the quest to decipher disease-associated biomarkers, miniaturized and multiplexed antibody arrays may play a central role in generating protein expression profiles, or protein maps, of crude serum samples. In this conceptual study, we explored a novel, 4-times larger pen design, enabling us to, in a unique manner, simultaneously print 48 different reagents (antibodies) as individual 78.5 μm(2) (10 μm in diameter) sized spots at a density of 38 000 spots cm(-2) using dip-pen nanolithography technology. The antibody array set-up was interfaced with a high-resolution fluorescent-based scanner for sensitive sensing. The performance and applicability of this novel 48-plex recombinant antibody array platform design was demonstrated in a first clinical application targeting SLE nephritis, a severe chronic autoimmune connective tissue disorder, as the model disease. To this end, crude, directly biotinylated serum samples were targeted. The results showed that the miniaturized and multiplexed array platform displayed adequate performance, and that SLE-associated serum biomarker panels reflecting the disease process could be deciphered, outlining the use of miniaturized antibody arrays for disease proteomics and biomarker discovery.

Detaljer

Författare
  • Linn Petersson
  • Linda Dexlin-Mellby
  • Anders Bengtsson
  • Gunnar Sturfelt
  • Carl A K Borrebaeck
  • Christer Wingren
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Reumatologi och inflammation
Originalspråkengelska
Sidor (från-till)1931-1942
TidskriftLab on a Chip
Volym14
Utgivningsnummer11
StatusPublished - 2014
PublikationskategoriForskning
Peer review utfördJa