Multiscale Characterization of Embryonic Long Bone Mineralization in Mice

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Abstract

Long bone mineralization occurs through endochondral ossification, where a cartilage template mineralizes into bone-like tissue with a hierarchical organization from the whole bone-scale down to sub-nano scale. Whereas this process has been extensively studied at the larger length scales, it remains unexplored at some of the smaller length scales. In this study, the changes in morphology, composition, and structure during embryonic mineralization of murine humeri are investigated using a range of high-resolution synchrotron-based imaging techniques at several length scales. With micro- and nanometer spatial resolution, the deposition of elements and the shaping of mineral platelets are followed. Rapid mineralization of the humeri occurs over approximately four days, where mineral to matrix ratio and calcium content in the most mineralized zone reach adult values shortly before birth. Interestingly, zinc is consistently found to be localized at the sites of ongoing new mineralization. The mineral platelets in the most recently mineralized regions are thicker, longer, narrower, and less aligned compared to those further into the mineralized region. In summary, this study demonstrates a specific spatial distribution of zinc, with highest concentration where new mineral is being deposited and that the newly formed mineral platelets undergo slight reshaping and reorganization during embryonic development.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Imperial College London
  • University of Eastern Finland
  • European Synchrotron Radiation Facility
  • Chalmers Tekniska Högskola
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Geokemi

Nyckelord

Originalspråkengelska
Artikelnummer2002524
TidskriftAdvanced Science
Volym7
Utgåva nummer21
StatusPublished - 2020 nov 1
PublikationskategoriForskning
Peer review utfördJa