Near-Edge Soft X-ray Absorption Mass Spectrometry of Protonated Melittin

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title = "Near-Edge Soft X-ray Absorption Mass Spectrometry of Protonated Melittin",
abstract = "We have investigated the photoionization and photofragmentation yields of gas-phase multiply protonated melittin cations for photon energies at the K-shell absorption edges of carbon, nitrogen, and oxygen. Two similar experimental approaches were employed. In both experiments, mass selected [melittin+qH]q+ (q=2–4) ions were accumulated in radiofrequency ion traps. The trap content was exposed to intense beams of monochromatic soft X-ray photons from synchrotron beamlines and photoproducts were analyzed by means of time-of-flight mass spectrometry. Mass spectra were recorded for fixed photon energies, and partial ion yield spectra were recorded as a function of photon energy. The combination of mass spectrometry and soft X-ray spectroscopy allows for a direct correlation of protein electronic structure with various photoionization channels. Non-dissociative single and double ionization are used as a reference. The contribution of both channels to various backbone scission channels is quantified and related to activation energies and protonation sites. Soft X-ray absorption mass spectrometry combines fast energy deposition with single and double ionization and could complement established activation techniques. [Figure not available: see fulltext.].",
keywords = "Melittin, Photodissociation, Protonated proteins, Soft X-ray absorption, Soft X-ray spectroscopy",
author = "Dmitrii Egorov and Sadia Bari and Rebecca Boll and Simon D{\"o}rner and Sascha Deinert and Simone Techert and Ronnie Hoekstra and Vicente Zamudio-Bayer and Rebecka Lindblad and Christine B{\"u}low and Martin Timm and {von Issendorff}, Bernd and Lau, {J. Tobias} and Thomas Schlath{\"o}lter",
year = "2018",
doi = "10.1007/s13361-018-2035-6",
language = "English",
volume = "29",
pages = "2138--2151",
journal = "Journal of the American Society for Mass Spectrometry",
issn = "1879-1123",
publisher = "Elsevier",
number = "11",