Near-Edge Soft X-ray Absorption Mass Spectrometry of Protonated Melittin

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Near-Edge Soft X-ray Absorption Mass Spectrometry of Protonated Melittin. / Egorov, Dmitrii; Bari, Sadia; Boll, Rebecca; Dörner, Simon; Deinert, Sascha; Techert, Simone; Hoekstra, Ronnie; Zamudio-Bayer, Vicente; Lindblad, Rebecka; Bülow, Christine; Timm, Martin; von Issendorff, Bernd; Lau, J. Tobias; Schlathölter, Thomas.

I: Journal of the American Society for Mass Spectrometry, Vol. 29, Nr. 11, 2018, s. 2138-2151.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Egorov, D, Bari, S, Boll, R, Dörner, S, Deinert, S, Techert, S, Hoekstra, R, Zamudio-Bayer, V, Lindblad, R, Bülow, C, Timm, M, von Issendorff, B, Lau, JT & Schlathölter, T 2018, 'Near-Edge Soft X-ray Absorption Mass Spectrometry of Protonated Melittin', Journal of the American Society for Mass Spectrometry, vol. 29, nr. 11, s. 2138-2151. https://doi.org/10.1007/s13361-018-2035-6

APA

Egorov, D., Bari, S., Boll, R., Dörner, S., Deinert, S., Techert, S., Hoekstra, R., Zamudio-Bayer, V., Lindblad, R., Bülow, C., Timm, M., von Issendorff, B., Lau, J. T., & Schlathölter, T. (2018). Near-Edge Soft X-ray Absorption Mass Spectrometry of Protonated Melittin. Journal of the American Society for Mass Spectrometry, 29(11), 2138-2151. https://doi.org/10.1007/s13361-018-2035-6

CBE

Egorov D, Bari S, Boll R, Dörner S, Deinert S, Techert S, Hoekstra R, Zamudio-Bayer V, Lindblad R, Bülow C, Timm M, von Issendorff B, Lau JT, Schlathölter T. 2018. Near-Edge Soft X-ray Absorption Mass Spectrometry of Protonated Melittin. Journal of the American Society for Mass Spectrometry. 29(11):2138-2151. https://doi.org/10.1007/s13361-018-2035-6

MLA

Vancouver

Author

Egorov, Dmitrii ; Bari, Sadia ; Boll, Rebecca ; Dörner, Simon ; Deinert, Sascha ; Techert, Simone ; Hoekstra, Ronnie ; Zamudio-Bayer, Vicente ; Lindblad, Rebecka ; Bülow, Christine ; Timm, Martin ; von Issendorff, Bernd ; Lau, J. Tobias ; Schlathölter, Thomas. / Near-Edge Soft X-ray Absorption Mass Spectrometry of Protonated Melittin. I: Journal of the American Society for Mass Spectrometry. 2018 ; Vol. 29, Nr. 11. s. 2138-2151.

RIS

TY - JOUR

T1 - Near-Edge Soft X-ray Absorption Mass Spectrometry of Protonated Melittin

AU - Egorov, Dmitrii

AU - Bari, Sadia

AU - Boll, Rebecca

AU - Dörner, Simon

AU - Deinert, Sascha

AU - Techert, Simone

AU - Hoekstra, Ronnie

AU - Zamudio-Bayer, Vicente

AU - Lindblad, Rebecka

AU - Bülow, Christine

AU - Timm, Martin

AU - von Issendorff, Bernd

AU - Lau, J. Tobias

AU - Schlathölter, Thomas

PY - 2018

Y1 - 2018

N2 - We have investigated the photoionization and photofragmentation yields of gas-phase multiply protonated melittin cations for photon energies at the K-shell absorption edges of carbon, nitrogen, and oxygen. Two similar experimental approaches were employed. In both experiments, mass selected [melittin+qH]q+ (q=2–4) ions were accumulated in radiofrequency ion traps. The trap content was exposed to intense beams of monochromatic soft X-ray photons from synchrotron beamlines and photoproducts were analyzed by means of time-of-flight mass spectrometry. Mass spectra were recorded for fixed photon energies, and partial ion yield spectra were recorded as a function of photon energy. The combination of mass spectrometry and soft X-ray spectroscopy allows for a direct correlation of protein electronic structure with various photoionization channels. Non-dissociative single and double ionization are used as a reference. The contribution of both channels to various backbone scission channels is quantified and related to activation energies and protonation sites. Soft X-ray absorption mass spectrometry combines fast energy deposition with single and double ionization and could complement established activation techniques. [Figure not available: see fulltext.].

AB - We have investigated the photoionization and photofragmentation yields of gas-phase multiply protonated melittin cations for photon energies at the K-shell absorption edges of carbon, nitrogen, and oxygen. Two similar experimental approaches were employed. In both experiments, mass selected [melittin+qH]q+ (q=2–4) ions were accumulated in radiofrequency ion traps. The trap content was exposed to intense beams of monochromatic soft X-ray photons from synchrotron beamlines and photoproducts were analyzed by means of time-of-flight mass spectrometry. Mass spectra were recorded for fixed photon energies, and partial ion yield spectra were recorded as a function of photon energy. The combination of mass spectrometry and soft X-ray spectroscopy allows for a direct correlation of protein electronic structure with various photoionization channels. Non-dissociative single and double ionization are used as a reference. The contribution of both channels to various backbone scission channels is quantified and related to activation energies and protonation sites. Soft X-ray absorption mass spectrometry combines fast energy deposition with single and double ionization and could complement established activation techniques. [Figure not available: see fulltext.].

KW - Melittin

KW - Photodissociation

KW - Protonated proteins

KW - Soft X-ray absorption

KW - Soft X-ray spectroscopy

U2 - 10.1007/s13361-018-2035-6

DO - 10.1007/s13361-018-2035-6

M3 - Article

C2 - 30047073

AN - SCOPUS:85054893150

VL - 29

SP - 2138

EP - 2151

JO - Journal of the American Society for Mass Spectrometry

JF - Journal of the American Society for Mass Spectrometry

SN - 1879-1123

IS - 11

ER -