NMR solution structure of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea

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Abstract

The structure of calerythrin, a prokaryotic 20 kDa calcium-binding protein has been determined by solution NMR spectroscopy. Distance, dihedral angle, J coupling, secondary chemical shift, residual dipolar coupling and radius of gyration restraints reveal four EF-hand motifs arranged in a compact globular structure. A tight turn in the middle of the amino acid sequence brings the two halves, each comprising a pair of EF-hands, close together. The structural similarity between calerythrin and the eukaryotic sarcoplasmic calcium-binding proteins is notable.

Detaljer

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Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysikalisk kemi

Nyckelord

Originalspråkengelska
Sidor (från-till)2505-2512
TidskriftEuropean Journal of Biochemistry
Volym270
Utgåva nummer11
StatusPublished - 2003
PublikationskategoriForskning
Peer review utfördJa